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Identificação de fontes de resistência em cultivares de milho à spodoptera frugiperda (J. E. Smith, 1797) em casa-de-vegetação BABT
Silva,Deise Maria P. da; Oliveira,José V. de; Tabosa,José Nildo; Barros,Reginaldo; Santos,Elton O. dos; Azevedo,Simone S..
Dois experimentos foram estudados em Casa-de-Vegetação, com o objetivo de identificar fontes de resistência em 15 cultivares de milho à Spodoptera frugiperda. Utilizaram-se as cultivares CMS-35, CMS-36, Dina-70, Cargill-525, Pioneer-3210, Pioneer-6875, BR-201, BR-451, AG-106, AG-510, CMS-22, Contimax-322, Jatinã C3 Anão, Cargill-511 e BR-106. As plantas foram infestadas com 30 lagartas recém eclodidas por planta, quando estas apresentavam de 6 a 8 folhas. Após 10 e 14 dias da infestação, foram avaliados os danos através de uma escala visual de notas de 0 a 9. Não houve diferença estatística significativa entre as cultivares para os parâmetros estudados: dano, peso, comprimento e sobrevivência das lagartas. No entanto, as correlações lineares entre o peso x...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Resistance; Zea mays; Foliar Damage; Fall armyworm; Larvae weight.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100014
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Impact of the number of Spodoptera frugiperda egg layers on parasitism by Trichogramma atopovirilia Scientia Agricola
Beserra,Eduardo Barbosa; Parra,José Roberto Postali.
Egg parasitoids of the genus Trichogramma (Hymenoptera: Trichogrammatidae) can be found in several crops attacking Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) eggs. It is therefore necessary to demonstrate the capacity of these natural enemies in suppressing populations of the pest to allow them to be used in biological control programs against that species. This work had the objective of evaluating the impact of egg layer distribution in S. frugiperda egg masses on the parasitism capacity of Trichogramma atopovirilia Oatman & Platner, 1983. Masses containing one, two, and three layers were used as treatments, and 1.6 parasitoid per egg of the pest were released. Parasitism percentage differences were observed among the three...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Insecta; Fall armyworm; Egg parasitoid; Biological control.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200016
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In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines PAB
Sihler,William; Souza,Marlinda Lobo de; Valicente,Fernando Hercos; Falcão,Rosana; Sanches,Marcio Martinello.
Abstract: The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Baculovirus; Biological control; Cell culture; Fall armyworm; In vitro production; SfMNPV.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100001
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Influence of parasitism by Chelonus insularis Cresson (Hymenoptera: Braconidae) on the susceptibility of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) to insecticides Neotropical Entomology
Zenner,Ingeborg; Álvarez,Alonso; Barreto,Sandra.
The egg-larval parasitoid Chelonus insularis Cresson is a key parasitoid of the fall armyworm Spodoptera frugiperda (J. E. Smith) during the second field generation in the upper Magdalena River Basin, Colombia. In spite of selection pressure, the higher susceptibility of the second generation fall armyworm larvae to insecticides, compared with that of the first generation, suggests that the parasitism may be responsible for the apparent difference in susceptibility. Parasitized and non-parasitized 2nd-instar larvae of the fall armyworm were tested for susceptibility to chlorpyriphos, methomyl, cypermethrin, and Bacillus thuringiensis in the laboratory, using the leaf dip test. Parasitized larvae were up to 3.93 times more susceptible to chlorpyriphos, 3.71...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fall armyworm; Egg-larval parasitoid; Insecticide resistance; Integrated pest management.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600015
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Influência de cultivar e nível de infestação de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) no rendimento do sorgo Anais da SEB
Cortez,Mirko G. R.; Waquil,José M..
An experiment was conducted in the greenhouse to evaluate the effect of sorghum (Sorghum bicolor) cultivars and Spodoptera frugiperda (J. E. Smith) infestations, at different plant developmental stages, on sorghum yield. Second instar larvae of S. frugiperda were used to infest plants. Significant differences (P ≤ 0,05) were found for the factors cultivar and stage of infestation for panicle (size and weight), grain (volume and weight) and 100 grain weight. No significant differences were observed for grain yield between damaged plant and the checks for the cvs. AG 3001 and CMSXS 375. However, the yield loss for the cv. BR 300 ranged from 11,6 to 21,7% for plants infested at 1st (15 days) and 4th stage (15 and 37 days), respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Fall armyworm; Pest management; Sorghum bicolor; Plant resistance; Pest damage.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000200028
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Insecticidal activity of culture supernatants from Bacillus thuringiensis Berliner strains against Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) larvae Anais da SEB
Barreto,Marliton R.; Loguercio,Leandro L.; Valicente,Fernando H.; Paiva,Edilson.
Novel vegetative insecticidal proteins (Vips) identified in the supernatant of Bacillus thuringiensis (B.t.) cultures have shown to provide adequate control over a wide spectrum of economically important crop pests. To evaluate the potential applicability of these proteins against fall armyworm (Spodoptera frugiperda Smith) larvae, the most important insect pest for tropical maize, the characteristics and mortality effects of culture supernatants from five B.t. strains were investigated. Striking differences among strains were detected, not only in terms of efficiency in killing the insect, but also regarding to mortality effects of heated and non-heated supernatants, which were used to distinguish the heat-sensitive protein-derived insecticidal fraction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Biological control; Fall armyworm; Vegetative insecticidal proteins; Vip genes.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000400010
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Insecticidal and growth inhibiting action of the supercritical extracts of Melia azedarach on Spodoptera frugiperda AGRIAMBI
Scapinello,Jaqueline; Oliveira,José V. de; Chiaradia,Luis A.; Tomazelli Junior,Osmar; Niero,Rivaldo; Dal Magro,Jacir.
This study evaluates the effects of supercritical carbon dioxide extracts obtained from the fruit of Melia azedarach L. (Meliaceae) on the development of Spodoptera frugiperda J.E. Smith, 1797 (Lepidoptera: Noctuidae), from the larval period until the insect adulthood. For this purpose, a laboratory-scale extraction unit was operated at 60 °C and 200 bar. Larval mortality increased by raising the extract concentration tested: the value of LC50 calculated by Probit analysis was 376.74 mg kg-1, and at a concentration of 5000 mg kg-1, the mortality was 100%. The extract exhibited insect growth inhibitory activity in the higher concentrations (500 mg kg-1, 1000 mg kg-1 and 5000 mg kg-1), due to antifeedant action, resulting in larvae and pupae with mass and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Supercritical extraction; Fall armyworm; Chinaberry.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662014000800014
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Larval development of Spodoptera eridania and Spodoptera frugiperda fed on fresh ear of field corn expressing the Bt proteins (Cry1F and Cry1F + Cry1A.105 + Cry2Ab2) Ciência Rural
Bortolotto,Orcial Ceolin; Bueno,Adeney de Freitas; Queiroz,Ana Paula de; Silva,Gabriela Vieira.
ABSTRACT: The objective of this study was to evaluate extent of larval period, larval survival (%), food consumption, and pupal biomass of Spodoptera eridania and Spodoptera frugiperda (Lepidoptera: Noctuidae ) fed on fresh ears of field corn expressing Bt proteins (Cry1F and Cry1F+Cry1A.105+Cry2Ab2). Larvae of Spodoptera spp. survived less than two days when they consumed Bt corncobs and showed 100% mortality. Spodoptera eridania reared on non-Bt corn cobs showed higher larval development (21.6 days) than S. frugiperda (18.4 days) and lower viability (56.4% and 80.2% for S. eridania and S. frugiperda , respectively). A higher amount of corn grains was consumed by S. eridania (5.4g) than by S. frugiperda (3.9g). In summary, this study demonstrated that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biology; Integrated pest management; Fall armyworm; Southern armyworm; Varietal resistance.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101898
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Metodologia para avaliação do efeito de inseticidas sobre Spodoptera frugiperda (J.E. Smith) na cultura do arroz irrigado Anais da SEB
Martins,José F. S.; Loeck,Alei E.; Botton,Marcos; Carbonari,Jairo J.; Gusmão,Luciana G..
A new methodology to evaluate the effect of insecticides on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in flooded rice (Oryza saliva) was tested. Artificial infestations with fall armyworm larvae (3rd -4th instars) on rice field plots, fenced by metallic cages was used. Larval mortality was evaluated 48 hours after insecticide application by flooding the rice field and counting the floating surviving insects. Lambdacyalothrin CE (7.5 g/ha) and permethrin CE (25 g/ha) provided high larval control (> 95 %). Cifluthrin CE (7.5 g/ha) and deltamethrin CE (2.0 and 2.5 g/ha) caused less mortality (< 70%). The advantages of this new methodology are discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Fall armyworm; Chemical control.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100025
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Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops PAB
Queiroz,Paulo Roberto; Ramiro,Carolina Almeida; Martins,Érica Soares; Soberón,Mário; Bravo,Alejandra; Monnerat,Rose Gomes.
Abstract: The objective of this work was to analyze the genetic variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations collected from corn and cotton crops in Brazil. The samples were analyzed by DNA markers. The dendrogram produced by the 20 RAPD primers evaluated showed a correlation between genetic profile and feeding behavior. The analysis of the mitochondrial ND1 gene allowed identifying the insect populations in both crops, and, in corn, in several geographical regions. The presented strategy allows the identification of Spodoptera frugiperda populations associated with corn and cotton crops.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Zea mays; Fall armyworm; Haplotypes; ND1 gene; RAPD..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500692
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New method for rearing Spodoptera frugiperda in laboratory shows that larval cannibalism is not obligatory Rev. Bras. entomol.
Silva,Cherre Sade Bezerra Da; Parra,José Roberto Postali.
New method for rearing Spodoptera frugiperda in laboratory shows that larval cannibalism is not obligatory. Here we show, for the first time, that larvae of the fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera, Noctuidae), can be successfully reared in a cohort-based manner with virtually no cannibalism. FAW larvae were reared since the second instar to pupation in rectangular plastic containers containing 40 individuals with a surprisingly ca. 90% larval survivorship. Adult females from the cohort-based method showed fecundity similar to that already reported on literature for larvae reared individually, and fertility higher than 99%, with the advantage of combining economy of time, space and material resources. These findings suggest that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial diet; Cannibalistic behavior; Fall armyworm; Mass production; Rearing technique.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000300015
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Nitrogen fertilization sources and insecticidal activity of aqueous seeds extract of Carica papaya against Spodoptera frugiperda in maize Ciencia e Investigación Agraria
Figueroa-Brito,Rodolfo; Villa-Ayala,Patricia; López-Olguín,Jesús F; Huerta-de la Peña,Arturo; Pacheco-Aguilar,Juan R; Ramos-López,Miguel A.
R. Figueroa-Brito, P. Villa-Ayala, J.F. López-Olguín, A. Huerta-de la Pena, J.R. Pacheco-Aguilar, and M.A. Ramos-López. 2013. Nitrogen fertilization sources and insecticidal activity of aqueous seeds extract of Carica papaya against Spodoptera frugiperda in maize. Cien. Inv. Agr. 40(3): 567-577. The damage caused by the fall armyworm Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) to vegetative-stage maize cultivated with chemical nitrogen fertilizers, vermicompost, and Carica papaya L. (Caricaceae) seed extract was estimated. Each shoot was infested with one first instar larva of S. frugiperda. The variables measured included the percentage of germination, length of the second and fifth leaves, stem diameter and plant height, and the estimation of...
Tipo: Journal article Palavras-chave: Aqueous extract; Caricaceae; Chemical fertilizers; Damage; Fall armyworm; Spodoptera frugiperda.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300009
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Ocimum basilicum essential oil combined with deltamethrin to improve the management of Spodoptera frugiperda Ciência e Agrotecnologia
Silva,Sérgio Macedo; Cunha,João Paulo Arantes Rodrigues da; Carvalho,Stephan Malfitano de; Zandonadi,César Henrique Souza; Martins,Rafael Castro; Chang,Roberto.
ABSTRACT For an important and expensive crop such as corn, the resistance of Spodoptera frugiperda J.E. Smith to various pesticides has led to research throughout the world for a potential insecticide from a natural source. For the management of pest resistance, natural compounds associated with synthetic insecticides can be a promising tool because they can reduce the application of the synthetics molecules while maintaining their effectiveness and promoting the control of the pests. Linalool is a potential insecticide that is easily obtained because it is found in high concentrations in the essential oil of Ocimum basilicum L. Therefore, the present study aimed to evaluate the toxicity of this essential oil and its combination with deltamethrin to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural insecticides; Linalool; Fall armyworm; Pyrethroid; Synergism..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000600665
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Oviposição, desenvolvimento e reprodução de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) em diferentes hospedeiros de importância econômica Neotropical Entomology
Barros,Eduardo M; Torres,Jorge B; Bueno,Adeney F.
The host selection for oviposition by Spodoptera frugiperda (J.E. Smith) among corn, millet, cotton and soybean, and its relationship with the biological characteristics were investigated. Free and non-choice tests for oviposition using plots containing five plants each, from each host in plastic greenhouse, resulted in similar oviposition preference among the host plants. In addition, selected biological characteristics of S. frugiperda were determined in the laboratory with larvae feeding on host leaves, and the combination of leaf and cotton boll. Neonate larvae exhibited low success of colonization on cotton boll compared to the leaves of all other hosts. Spodoptera frugiperda fed only on cotton bolls exhibited longer larval and pupal development, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fall armyworm; Oviposition behavior; Host selection; Fertility life table.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000600023
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Parasitism capacity of Telenomus remus Nixon (Hymenoptera: Scelionidae) on Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) eggs BABT
Bueno,Regiane Cristina Oliveira de Freitas; Carneiro,Tatiana Rodrigues; Bueno,Adeney de Freitas; Pratissoli,Dirceu; Fernandes,Odair Aparecido; Vieira,Simone Silva.
This work studied the parasitism capacity of Telenomus remus Nixon (Hymenoptera: Scelionidae) on Spodoptera frugiperda (Smith) (Hymenoptera: Scelionidae) eggs at 15, 20, 25, 28, 31, and 35°C, aiming to use this natural enemy in biological control programs in crops where S. frugiperda was considered pest. The parasitism during the first 24 h was 60.90, 81.65, 121.05, 117.55 and 108.55 parasited eggs per female from egg masses of approximately 150 eggs, at 15, 20, 25, 28 and 31°C, respectively. Females of T. remus reached parasitism higher than 80% at 15, 20, 25, 28 and 31ºC at 5, 27, 8, 2, and 2 days, respectively. At 35ºC, there was no parasitism. The highest parasitism rates occurred at 20, 25, 28 and 31°C. T. remus female longevity varied from 15.7 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eggs parasitoid; Fall armyworm; Biological control; Integrated Pest Management.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100017
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Population fluctuation of Spodoptera frugiperda eggs and natural parasitism by Trichogramma in maize - doi: 10.4025/actasciagron.v35i3.16769 Agronomy
Bastos Dequech, Sônia Thereza; Universidade Federal de Santa Maria; Camera, Cátia; Universidade Federal de Santa Maria; Sturza, Vinicius Soares; Universidade Federal de Santa Maria; Ribeiro, Leandro do Prado; Universidade de São Paulo; Querino, Ranyse Barbosa; Empresa Brasileira de Pesquisa Agropecuária; Poncio, Sônia; Universidade Federal de Santa Maria.
This study aimed to evaluate the fluctuation of S. frugiperda eggs and natural parasitism by Trichogramma and identify and quantify parasitoid species in maize fields in the state of Rio Grande do Sul, Brazil. Surveys were carried out in two growing seasons: in 2006/2007 we assessed one growing season (late sowing) in Santa Maria, and in 2007/2008 we assessed two growing seasons (early and late sowing) in Santa Bárbara do Sul. Daily air temperature and rainfall were recorded at both locations. In each evaluation, entire plants were examined for the presence or absence of S. frugiperda egg masses, which were analyzed for larvae hatching or parasitoid emergence. The number of S. frugiperda eggs is higher between 8 and 30 days after plant emergence,...
Palavras-chave: Agronomia; Fitossanidade biological control; Fall armyworm; Noctuidae; Trichogrammatidae; Zea mays L. entomologia agrícola.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16769
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Preference of Neoseiulus californicus (Acari: Phytoseiidae) for volatiles of Bt maize induced by multiple herbivory Rev. Bras. entomol.
Botti,Jéssica Mayara Coffler; Franzin,Mayara Loss; Fadini,Marcos Antonio Matiello; Melo,Júlio Onésio Ferreira.
ABSTRACT Plant indirect induced defenses against herbivores are characterized by the production of plant volatiles that to attract natural enemies. The objective of this study was to evaluate whether the attack of the two-spotted spider mite Tetranychus urticae or the multiple herbivory of T. urticae together with the fall armyworm Spodoptera frugiperda are able to elicit indirect induced defense in conventional and Bt maize plants. The experiment was carried out in the laboratory using Y-tube olfactometer, evaluating the predatory mite Neoseiulus californicus olfactory preference for plant volatiles. The treatments involved: Clean Conventional Plant; Conventional Plant Infested with T. urticae; Clean Bt Plant; Bt Plant Infested with T. urticae;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indirect induced defense; Tetranychus urticae; Secondary plant compounds; Plant resistance; Fall armyworm.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000400283
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Spatial analysis of the distribution of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) and losses in maize crop productivity using geostatistics Neotropical Entomology
Farias,Paulo R.S.; Barbosa,José C.; Busoli,Antonio C.; Overal,William L.; Miranda,Vicente S.; Ribeiro,Susane M..
The fall armyworm, Spodoptera frugiperda (J.E. Smith), is one of the chief pests of maize in the Americas. The study of its spatial distribution is fundamental for designing correct control strategies, improving sampling methods, determining actual and potential crop losses, and adopting precise agricultural techniques. In São Paulo state, Brazil, a maize field was sampled at weekly intervals, from germination through harvest, for caterpillar densities, using quadrates. In each of 200 quadrates, 10 plants were sampled per week. Harvest weights were obtained in the field for each quadrate, and ear diameters and lengths were also sampled (15 ears per quadrate) and used to estimate potential productivity of the quadrate. Geostatistical analyses of caterpillar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Fall armyworm; Semivariogram; Kriging.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000300012
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The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae) Rev. Bras. entomol.
Roel,Antonia Railda; Dourado,Doroty M.; Matias,Rosemary; Porto,Karla R. A.; Bednaski,Aline V.; Costa,Reginaldo B. da.
The fall armyworm, Spodoptera frugiperda, is one of the major field pests for maize production. It is mainly controlled by means of synthetic, and more recently by resistant cultivar of maize expressing Bt toxins. The neem tree, Azadirachta indica, is a plant that can potentially control insects with the advantage of being food and environmental safe. The aim of this study was to assess the effect of neem oil on the development and survival of S. frugiperda caterpillars by assessing histological alterations caused on their midgut. Newly hatched caterpillars were submitted to three neem oil concentrations: 0.006; 0.05; 0.4%, which were added to their artificial diet. Ten 3rd instar caterpillars, taken from each treatment, were submitted to histological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fall armyworm; Histological analysis; Insecticidal plants; Neem.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262010000300024
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Use of serological techniques for determination of Spodoptera frugiperda(J E Smith) predators (Lepidoptera: Noctuidae) Neotropical Entomology
Santos-Neto,Joaquim R; Mezencio,Jose M S; Chagas,Aline T A; Michereff-Filho,Miguel; Serrão,José E.
Spodoptera frugiperda (J E Smith) is an important pest of several crops, but especially on maize in Brazil. The implementation of biological control measures hinges on the identification of its predators and other natural enemies. As a means of identifying predators, antibodies against S. frugiperda eggs were generated by inoculating rabbits with macerated S. frugiperda eggs, and the production of antibodies against S. frugiperda egg proteins was verifi ed by double immunodiffusion (DID). These antibodies were then utilized in another serological technique, counterimmunoeletrophoresis (CIE), to identify insects that could have ingested S. frugiperda eggs. Macerates of entire insects collected in maize plantations and of individual parts of their digestive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fall armyworm; Natural enemy; Double immunodiffusion; Counterimmunoelectrophoresis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000300016
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